Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 1. P ®mbriae augment epithelial cytokine response of A498 cells to E. coli (108 cfu mlÿ1). Supernatants were collected at 0, 2, 6 and 24 h. Concentrations of IL-6 (A) and IL-8 (B) were determined by ELISA. Numbers are means 6 s.e.m. of ®ve experiments.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 2. Top. msbB and pap genotypes of the E. coli strains used in the present study as shown by colony hybridization. The msbB gene was detected using the 1630 bp msbB DNA fragment as a probe. The pap sequences were detected by hybridization with the 5 kb HindIII fragment of the pap gene cluster from E. coli J96. Bottom. The E. coli strains JM83 (msbB) and M607 (msbB±) were transformed with pPIL110- 75. Con¯uent human kidney A498 cells were exposed to the ®mbriated strains as well as the non-®mbriated isogenic strains (108 cfu mlÿ1). IL-6 (A) and IL-8 (B) concentrations were measured by ELISA in supernatants after 0, 2, 6 and 24 h of stimulation. Numbers are means 6 s.e.m. of seven experiments. Transduction of P-®mbriated E. coli with the non-functional msbB gene from E. coli M607 does not alter epithelial cytokine responses to this strain. strr/spcr E. coli AD110 was used for cell activation experiments. Con¯uent layers of A498 cells were stimulated with E. coli AD110 (msbB) and E. coli AD110 (msbB±) (108 cfu mlÿ1). Samples collected at 0, 2, 6 and 24 h was used for IL-6 (C) and IL-8 (D) determinations. Numbers are means 6 s.e.m. of seven experiments.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Hybridization, Transformation Assay, Enzyme-linked Immunosorbent Assay, Transduction, Functional Assay, Activation Assay

Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 3. The LPS inhibitor BPI does not reduce the cytokine response of A498 cells to E. coli. Con¯uent layers of A498 cells were exposed to E. coli HB101(pPIL110-75) (108 cfu mlÿ1) in the presence or absence of BPI (10 nM, 100 nM, 1 mM). Samples were collected after 0, 0.5, 2, 4, 6 and 24 h. IL-6 (A) and IL-8 (B) concentrations in the 6 h sample were determined by ELISA. Data represent means 6 s.e.m. of four experiments and triplicate samples.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 4. The anti-LPS drug Polymyxin B failed to reduce cytokine responses to whole bacteria. Con¯uent layers of A498 cells were exposed to E. coli HB101(pPIL110-75) (108 cfu mlÿ1) with or without Polymyxin B (50 ng mlÿ1, 500 ng mlÿ1, 5 mg mlÿ1, 50 mg mlÿ1). Samples were collected after 0, 0.5, 2, 4, 6 and 24 h. IL-6 (A) and IL-8 (B) concentrations in the 6 h samples are shown. The phorbol ester PMA was used as a non-bacterial stimulant to control non-speci®c effects of Polymyxin B. Data are means 6 s.e.m. of seven experiments and triplicate samples.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Bacteria, Control

Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 5. Cytokine response of A498 cells to smooth Salmonella LPS. Supernatants were collected at 0, 2, 6 and 24 h, and IL-6 (A) and IL-8 (B) concentrations were determined by ELISA. Results are means 6 s.e.m. of four experiments and triplicate samples.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 6. Surface CD14 expression as shown by ¯ow cytometry after staining with anti-CD14 antibody. Vitamin D3-treated U937 cells stained with anti-CD14 antibody but did not react with the control antibody. A498 cells failed to express surface CD14.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Expressing, Cytometry, Staining, Control

Journal: Molecular microbiology

Article Title: P fimbriae-dependent, lipopolysaccharide-independent activation of epithelial cytokine responses.

doi: 10.1046/j.1365-2958.1999.01513.x

Figure Lengend Snippet: Fig. 7. RT-PCR of CD14 mRNA in A498 and U937 cells. A. CD14 mRNA was detected in U937 cells stimulated with vitamin D3 for 48 h but not in A498 cells before or after 24 h of stimulation with LPS or E. coli AD110. B. RT-PCR of actin mRNA in the A498 and the U937 cells. Actin mRNA was detected in all cells.

Article Snippet: The human epithelial cell line A498 (ATCC HTB44, human kidney carcinoma) has previously been used as a model system in studies of bacterially induced epithelial cell responses to uropathogenic E. coli.

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Molecules

Article Title: Comparative Analysis of the Antitumor Activity of Cis- and Trans-Resveratrol in Human Cancer Cells with Different p53 Status

doi: 10.3390/molecules26185586

Figure Lengend Snippet: Tumor entities and p53 status of tumor cell lines employed throughout this study.

Article Snippet: Human hepatoma cell lines HepG2 (hepatoblastoma, DSMZ-No: ACC 180) and Hep3B (hepatocellular carcinoma, DSMZ-No: ACC 93), human colon carcinoma cell line HCT-116 (DSMZ-No: ACC 581), and human kidney carcinoma cell line A498 (DSMZ-No: ACC 55) were obtained from the German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany).

Techniques: Mutagenesis

Journal:

Article Title: Taxol Suppresses Dynamics of Individual Microtubules in Living Human Tumor Cells

doi:

Figure Lengend Snippet: Inhibition of proliferation of Caov-3 ovarian carcinoma cells (○) and A-498 kidney carcinoma cells (●) by taxol (24 h). Cell proliferation was determined by counting live cells at the time of taxol addition and 24 h later. Values >100% inhibition of proliferation indicate net loss of cells over the 24-h duration of taxol incubation. Values are means and SEs of 11 independent experiments for A-498 cells and 4 experiments for Caov-3 cells.

Article Snippet: Cell Culture and Proliferation A-498 1 epithelial-like human kidney carcinoma cells (ATCC HTB 44) were grown in 90% MEM, supplemented with 1.0 mM sodium pyruvate; Caov-3 epithelial-like human ovarian adenocarcinoma cells (ATCC HTB 75) were grown in 90% DMEM, supplemented with 4.5 g/l glucose.

Techniques: Inhibition, Incubation

Journal:

Article Title: Taxol Suppresses Dynamics of Individual Microtubules in Living Human Tumor Cells

doi:

Figure Lengend Snippet: Intracellular taxol concentration in human tumor cells after incubation for 24 h (or 4 h where noted) in taxol-containing medium and effects on proliferation

Article Snippet: Cell Culture and Proliferation A-498 1 epithelial-like human kidney carcinoma cells (ATCC HTB 44) were grown in 90% MEM, supplemented with 1.0 mM sodium pyruvate; Caov-3 epithelial-like human ovarian adenocarcinoma cells (ATCC HTB 75) were grown in 90% DMEM, supplemented with 4.5 g/l glucose.

Techniques: Concentration Assay, Incubation